Hideto Nakamura, Ken Sawada, Hiroya Araie, Iwane Suzuki, Yoshihiro Shiraiwa
Researches in Organic Geochemistry, Vol. 66, 90-97, 2014
Publication year: 2014

Highlights

  • Cultured Chrysotila lamellosa CCMP1307 produced high proportion of C37:4 alkenones.

  • C. lamellosa U37K values reproduced the typical values for C37:4-rich lakes.

  • UK37 calibration showed a better fit with temperature than UK′37 calibration.

  • The unsaturation degree of C31 alkenes was also controlled by growth temperature.

Abstract

The compositions of long chain alkenes, alkenones and alkenoates in a cultured strain of the haptophyte Chrysotila lamellosa CCMP1307, isolated from a salt marsh, were investigated. The biomarker patterns were distinctive and showed a high proportion of tetraunsaturated alkenones and alkenoates, with a pronounced proportion of C40 alkenones and a lack of C38 methyl and C39 ethyl alkenones. Linear regression of the alkenone unsaturation degree (UK37) with growth temperature (T) was obtained over the range of possible CCMP1307 growth temperature values (4–20 °C): UK37 = 0.045 × T (°C) − 1.016, (n = 13, r2 0.96), while the UK′37 values were weakly correlated with TUK′37 = 0.0035T (°C) + 0.0511 (n = 18, r2 0.70). The UK37 calibration exhibited a low y-intercept in comparison with that of a Chinese inland lake strain reported previously. The data show significant intraspecific variation in UK37 for C. lamellosa between strains from different geographic origins. C. lamellosa CCMP1307 reproduced typical UK37 values observed in C37:4 rich lakes, especially at lower temperature (< 15 °C). In addition, we identified C29:2, C31:1, C31:2 and C31:3 alkenes, and found that the unsaturation degree of C31 alkenes (U31en), calculated with C31:1 and C31:2 alkenes, might also be a useful index of growth temperature for the haptophyte C. lamellosa.

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